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Lysostaphin: a breakthrough pharmaceutical against Staphylococcus aureus
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Bacteria which are resistant to many available antibiotics are an increasing danger for hospitalized patients. On request of Biosynexus Inc., NIZO food research has developed a process for the production and purification of the enzyme lysostaphin. This enzyme cleaves specific peptide bridges of the murein of sensitive target bacteria such as Staphylococcus aureus. This process includes two micro-filtration steps and one high pressure homogenization to release and concentrate the enzyme. Further purification was carried out by hydrophobic interaction chromatography leading to a more than 90% pure product. This product has been further purified under cGMP conditions and used for successful clinical phase 1 and 2 trials. For more info on the NIZO Gene Expression Services follow this link |
microfiltration, chromatography, concentration, purification
